The Inventory of Ongoing Federal Food Safety Research contains food safety
projects that were being carried out by Agriculture and Agri-Food Canada, the Canadian Food Inspection
Agency and Health Canada in 2003. This inventory is designed to provide
scientists, science managers and top executives with a global view of the food
safety research carried out in all federal institutions, in order to help
identify gaps in research, develop a national research strategy, and stimulate
collaboration.
The inventory was designed to be easy to use, with a short initial core
section intended for decision and policy makers consisting of a list of project
titles and a summary table, followed by appendices in which the projects are
described in more detail for use by scientists.
The inventory was also designed to be easy to update. Each project has been
given a unique alpha-numerical ID Project
Number, and this number will never change until the project is completed and
removed from the inventory. As an example, project number CFIA0215 is the fifteenth
Canadian Food Inspection Agency project entered in the inventory in the year
2002.
The projects are presented following the pattern:
Project number Project title
Project Leader: Name of
leading investigator, laboratory, E-mail address
Starting Date: Year/ Month.
(Only the starting date is given, since projects will be removed from the list
when completed)
Collaboration: Organizations other than the laboratory of the
project leader
Description: A concise description of the project goals
[maximum of 100 words]
Project Identification: Identification assigned by the
department or agency for tracking.
Top of Page
CFIA0201
Adaptation and evaluation of a gene probe method and a multiplex polymerase
chain reaction method for the detection and enumeration of Vibrio parahaemolyticus
CFIA0202 Improved
test capability for veterinary drug residues in dairy, eggs and honey
CFIA0204
Development/ implementation of analytical methods for the multi-residue
determination of chloramphenicol, florfenicol and thamphenicol in honey
CFIA0206
Development/ implementation of analytical method for the determination of
cephalosporins in milk
CFIA0207
Development/ implementation of analytical method for the determination of
coccidiostats in egg and egg products
CFIA0210
Development/ implementation of analytical method for the determination of
dipyrone, etodolac and metabolites in milk
CFIA0212
Development/ implementation of analytical method for the determination of
tetracyclines in egg and egg products
CFIA0213
Development/ implementation of an expansion in the scope of pesticides analyzed
in the pesticide multiresidue methods of analysis of fruit and vegetables
CFIA0214
Development/ implementation of analytical method for the determination of
abamectin in fruit and vegetable commodities
CFIA0215
Development/ implementation of analytical method for the determination of
imidacloprid in honey and its subsequent extension for the determination of
imidacloprid in fruit and vegetable commodities
CFIA0218
Development, optimization, validation and transfer of nucleic acid
sequence-based amplification and polymerase chain reaction techniques for the
detection and epidemiological characterization of food-borne viruses in foods
(shellfish, fruits, vegetables, meat products) and livestock
CFIA0220 Study of
protein expression profiles of Listeria
monocytogenes using two-dimensional electrophoresis for strain
identification, discrimination of the relative virulence potential and
epidemiological applications
CFIA0224 Low
density deoxyribonucleic acid and antibody arrays for the detection and
identification of foodborne pathogens
CFIA0235 Methods
for the isolation and detection of Cryptosporidium
in apple cider
CFIA0236
Establishment of accurate and rapid tests for food- and water-borne parasites:
Cyclospora, Giardia,
and Toxoplasma
CFIA0237
Standardization and validation of immunohistochemical and PCR assays for Cysticercus bovis
CFIA0243
Development of analytical methodology for the determination of synthetic
pyrethroid residues in tissue of slaughtered animals and in milk and dairy
products
CFIA0247 Improved
test capability for banned substances in food of animal origin
CFIA0248
Development and implementation of analytical methods for the determination of
carbadox and five other feed additives (spiramycin, tylosin, virginiamycin,
bacitracin and olaquindox) banned by the European Union for use as antimicrobial
and growth promotants in food animal production
CFIA0251
Development and implementation of multi-residue analytical methods for the
determination of Beta-lactams and phenicols in animal derived foods
CFIA0252
Development/ implementation of analytical methods for macrolide and
aminoglycoside antibiotics in foods of animal originals
CFIA0253
Development/ implementation of analytical methods for the detection of
prednisone and other corticosteroids in meat and meat products
CFIA0257
Evaluation of high through-put, rapid tests for bovine spongiform encephalopathy
(BSE)
CFIA0258
Enhancement and implementation of the Canada Database of Animal Parasites
CFIA0301 Mass
spectrometric verification of the endectocides doramectin, moxidectin,
abamectin, ivermectin, and eprinomectin in milk
CFIA0302
Development/ implementation of a multi-residue confirmatory method for the
determination of Beta-lactams or pencillins in eggs and milk using LC
MS/MS
CFIA0303
Development/ implementation of a multi-residue confirmatory method for the
determination of macrolides in eggs and milk using LC
MS/MS
CFIA0304
Development/ implementation of analytical methods for the detection of specific
fungicides in fruit and vegetable commodities
CFIA0305
Development of HPLC/MS
methodology for the determination of azaspiracids in shellfish
CFIA0306
Development of HPLC/MS
methodology for the determination of nitrofuran metabolites in aquacultured
products
CFIA0307 Carbon
monoxide detection in carbon monoxide-treated fish flesh
CFIA0308
Functional foods containing fish oils: Correlating sensory analysis with
volatile odour compounds
CFIA0309
Development and optimization of a nucleic acid sequence-based amplification
(NASBA)
"real-time" platform for the detection of hepatitis and Norwalk-like viruses in
foods
CFIA0310
Development, optimization and validation of a new PCR and culture procedures for the
detection and isolation of Shigella spp. in fresh produce
CFIA0311
Development, optimization and validation of molecular methods for the detection
of enteric viruses in bottled water and berries
CFIA0312
Confirmatory tests to corroborate milk allergen detection in various food
matrix
CFIA0313 Detection
of emerging zoonotic enteric viruses potentially transmitted through livestock
and food
CFIA0314
Evaluation of the suitability of a low density array method using a universal
sample preparation procedure for the detection of foodborne viruses using
DNA and RNA enteric viruses as models
CFIA0315
Evaluation and validation of the NASBA) amplification
approach for the detection of enteroviruses in food samples
CFIA0316 Rapid
detection and reduction of Salmonella Typhimurium
DT 104 in poultry using
bacteriophage
CFIA0317
Development of a novel, rapid and inexpensive method for epidemiological
analysis of Listeria monocytogenes and Escherichia coli strains isolated from clinical cases and
contaminated food during disease outbreaks
CFIA0318
Evaluation of postmortem diagnostic tests for confirmation of chronic wasting
disease in clinically affected and preclinical elk and deer
CFIA0319
Development of a bioassay to detect infectivity in tissues and body fluids of
elk and deer naturally infected with chronic wasting disease
CFIA0320
Preliminary investigation of host response patterns in chronic wasting disease
and scrapie as disease markers for preclinical diagnostic tests
CFIA0321
Development of ultrasensitive immuno-PCR and enzyme immuno-fluorescence
techniques for food testing: detection of bacterial toxins and quantitation of
peanut allergen in foods
CFIA0322
Validation of ELISA
for antemortem diagnosis of Cysticercus
bovis-infected cattle
CFIA0323 Improved
test capability for hormones and other growth promoting substances in urine and
tissues
CFIA0324
Development/ implementation of a multi-residue analytical method to determine
beta-lactams in honey using liquid chromatography/ electrospray ionization
tandem mass spectrometry
CFIA0325
Development/ implementation of analytical method to determine aminoglycosides in
honey
CFIA0326
Development/ implementation of analytical method for the determination of
tetracyclines in egg and egg products
Top of Page
CFIA0201
Adaptation and evaluation of a gene probe method and a multiplex polymerase
chain reaction method for the detection and enumeration of Vibrio parahaemolyticus
Project leader: Enrico A Buenaventura, Canadian Food Inspection
Agency-Burnaby, buenaventura@inspection.gc.ca
Starting date: 2001/04
Collaboration: Canadian Food Inspection Agency-Sidney
Description: Even though both pathogenic and non-pathogenic strains of
Vibrio parahaemolyticus occur naturally in the
environment and in molluscan shellfish including oysters, the current testing
methods do not distinguish between these strains. A multiplex detection system
for Vibrioparahaemolyticus will eliminate the
need to perform individual tests for each of the individual genotypes, reducing
the time and cost involved in confirmatory testing. This study will adapt and
evaluate new methods to detect and enumerate Vibrio
parahaemolyticus.
Project Identification: BB0101
CFIA0202
Improved test capability for veterinary drug residues in dairy, eggs and
honey
Project leader: Dugane Quon, Canadian Food Inspection Agency-Calgary,
quond@inspection.gc.ca
Starting date: 2000/04
Description: A need for better analysis for veterinary drug residues in
honey, eggs and dairy products was noted in the European Community audits of
Canada's Residue Control Programs for animal-derived foods in September 2000.
This study will enhance Canadian Food Inspection Agency test capabilities to
detect and analyze listed compounds in the residue control program.
Project Identification: CF0101
CFIA0204
Development/ implementation of analytical methods for the multi-residue
determination of chloramphenicol, florfenicol and thiamphenicol in
honey
Project leader: David Durden, Canadian Food Inspection Agency-Calgary,
durdend@inspection.gc.ca
Starting date: 2002/04
Description: There is a concern that the chloramphenicol and other phenicol
compounds may be present in honey. Objectives are to adapt and validate a
residue method for phenicols (chloramphenicol, florfenicol, thiamphenicol) in
honey, based upon the present chloramphenicol method in eggs and milk; develop a
more sensitive analytical method module for phenicols and their metabolites, for
screening or confirmation.
Project Identification: CF0202
CFIA0206
Development/ implementation of analytical method for the determination of
cephalosporins in milk
Project leader: David Durden, Canadian Food Inspection Agency-Calgary,
durdend@inspection.gc.ca
Starting date: 2002/04
Description: Cephalosporins, as part of the large group of Beta-lactam
antiobiotics, are widely used in agriculture, especially for treatment of
mastitis in lactating cattle. Canadian Food Inspection Agency has methods for
analysing ceftiofur residues in tissue. This method, however, has not been
transferred to other matrices such as milk or eggs. Following the European Union
audit report of 2000, several other cephalosporins, and possibly their
metabolites, were suggested to be added to the list of food residues. The
objectives are to adapt and validate a residue method for cephalosporins in
milk.
Project Identification: CF0204
CFIA0207
Development/ implementation of analytical method for the determination of
coccidiostats in egg and egg products
Project leader: Dugane Quon, Canadian Food Inspection Agency-Calgary,
quond@inspection.gc.ca
Starting date: 2002/04
Description: Two coccidiostats, nicarbazin and robenidine, are prohibited as
veterinary medical products for food producing animals in the European
Community. Canadian products must be tested to meet international levels for
export purposes. This study will expand on the capabilities of the Canadian Food
Inspection Agency's Canada National Chemical Residue Monitoring Program to
analyze for coccidiostats in eggs and egg products.
Project Identification: CF0205
CFIA0210
Development/ implementation of analytical method for the determination of
dipyrone, etodolac and metabolites in milk
Project leader: Gail White, Canadian Food Inspection Agency-Calgary,
whitegail@inspection.gc.ca
Starting date: 2002/04
Description: The United States Food and Drug Administration has banned
dipyrone for human use, and removed it from its list of approved veterinary
medications. The drug is also banned in Canada and many Western European
countries. Canadian Food Inspection Agency has a method for detecting dipyrone
and metabolites in bovine and porcine muscle. Since the milk matrix is somewhat
more complex than the tissue matrix, a new extraction procedure will have to be
developed. Objectives are to adapt and validate a residue method for dipyrone,
its metabolites and etodolac in milk.
Project Identification: CF0208
CFIA0212
Development/ implementation of analytical method for the determination of
tetracyclines in egg and egg products
Project leader: David Durden, Calgary, durdend@inspection.gc.ca
Starting date: 2002/04
Description: The Canadian Food Inspection Agency has quantified tetracyclines
in a variety of food stuff, and test methods have been developed in both Calgary
and Saskatoon. A method has been developed at the Calgary laboratory for
tetracycline residues (oxytetracycline, tetracycline, chlortetracycline) in
honey. It is proposed to extend this method to eggs and egg products. Objectives
are to adapt and validate a residue method for tetracyclines in eggs and egg
products and to add marker compounds to the residue method.
Project Identification: CF0210
CFIA0213
Development/ implementation of an expansion in the scope of pesticides analyzed
in the pesticide multi-residue methods of analysis of fruit and
vegetables
Project leader: Donna Grant, Canadian Food Inspection Agency-Calgary,
grantd@inspection.gc.ca
Starting date: 2002/04
Description: The pesticide multi-residue method in Canadian Food Inspection
Agency Calgary will screen for as many as 265 different pesticides in fruit and
vegetable matrices. Canada must expand the number of pesticides screened to
harmonize with the United States Department of Agriculture, and Food and Drug
Administration. This study will expand the scope of pesticides currently
available in the pesticide multi-residue analysis of fruit and vegetable
commodities.
Project Identification: CF0211
CFIA0214
Development/ implementation of analytical method for the determination of
abamectin in fruit and vegetable commodities
Project leader: Chris Tam, Canadian Food Inspection Agency-Calgary,
tamchris@inspection.gc.ca
Starting date: 2002/04
Description: To harmonize with the United States Department of Agriculture,
and Food and Drug Administration, there is a need to update the scope of
pesticides being monitored in Canada to include those monitored by American
government departments. This study will adapt and validate a residue method to
determine abamectin in fruit and vegetables.
Project Identification: CF0212
CFIA0215
Development/ implementation of analytical method for the determination of
imidacloprid in honey and its subsequent extension for the determination of
imidacloprid in fruit and vegetable commodities
Project leader: Chris Tam, Canadian Food Inspection Agency-Calgary,
tamchris@inspection.gc.ca
Starting date: 2002/04
Description: A major initiative of the Canadian Food Inspection Agency's
Calgary laboratory is to expand the pesticide multi-residue method, which
currently screens for as many as 265 different pesticides in fruit and vegetable
matrices. However, some pesticides would not be good candidates for inclusion in
this method. Hence, alternate methods are required for these pesticides. This
project will address those pesticides identified as being of high priority for
chemical residue monitoring. Objectives are to adapt and validate a residue
method to determine imidacloprid in honey, and extend and validate the method to
determine imidacloprid in fruit and vegetables.
Project Identification: CF0213
CFIA0217b
Development of sensory reference and training kits for fishing
industry
Project leader: D.G.McLachlan, Canadian Food Inspection Agency-Dartmouth,
mclachlang@inspection.gc.ca
Starting date: 1998/04
Description: This study will investigate the volatile chemical makeup of
several key fish and fish products, using gas chromatographic techniques to gain
the information necessary to develop and produce standardized olfactory
calibration reference kits. The standard calibration reference kits will
approximate the various sensory odours and intensities associated with fish,
from fresh caught to advanced decomposition.
Project Identification: FI9801
CFIA0218
Development, optimization, validation and transfer of nucleic acid
sequence-based amplification and polymerase chain reaction techniques for the
detection and epidemiological characterization of food-borne viruses in foods
(shellfish, fruits, vegetables, meat products) and livestock
Project leader: Carole Simard, Canadian Food Inspection Agency-St. Hyacinthe,
simardc@inspection.gc.ca
Starting date: 2000/04
Collaboration: Canadian Food Inspection Agency-Ottawa (Carling), Agriculture
and Agri-Food Canada: Food Research and Development Centre, Health Canada:
Bureau of Microbial Hazards - Food Directorate, Université Laval;
Intitute National de la Recherche Agronomique -Institut Armand
Frappier
Description: Assess the significance of foods in the transmission of enteric
viruses, as foods are likely to contain only low levels of viruses, adequate
concentration, purification and detection methods must be developed. This study
will investigate the development, optimization, validation and transfer of rapid
molecular techniques to detect, and epidemiologically characterize, food-borne
viruses in foods and livestock.
Project Identification: H0102
CFIA0220
Study of protein expression profiles of Listeria
monocytogenes using two-dimensional electrophoresis for strain
identification, discrimination of the relative virulence potential and
epidemiological applications
Project leader: Min Lin, Canadian Food Inspection Agency-Ottawa
(Fallowfield), linm@inspection.gc.ca
Starting date: 2002/04
Description: Whereas listeriosis is a rare human illness, it remains a
leading cause of mortality from a food-borne pathogen. A rapid detection method
for Listeria monocytogenes in perishable products,
including meat, poultry, processed egg and dairy products, and products which
have an inherently high background level of bacteria, is required. This study
examines a novel typing methodology for differentiating Listeria monocytogenes strains at an extremely high
resolution, and for assessing their virulence potentials.
Project Identification: NBR0219
CFIA0224
Low density DNA and antibody arrays for the detection and identification of
food-borne pathogens
Project leader: Burton W. Blais, Canadian Food Inspection Agency-Ottawa
(Carling), bblais@inspection.gc.ca
Starting date: 2002/09
Description: The development of a simple, rapid and inexpensive low density
array technology based on the use of macroporous hydrophobic cloth (polyester
cloth) as a solid support for the immobilization of DNA and antibody arrays is
proposed. Objectives are to develop simple DNA arrays capable of identifying
several key virulence, toxin and antibiotic resistance genes found in a variety
bacteria targeted by Canadian Food Inspection Agency food microbiology testing
programs; and an antibody array for use in serotyping Salmonella isolates.
Project Identification: OFL0201
CFIA0235
Methods for the isolation and detection of Cryptosporidium in apple cider
Project leader: Sarah Parker, Canadian Food Inspection Agency-Saskatoon,
sparker@inspection.gc.ca
Starting date: 2002/04
Description: Cryptosporidium is a water-borne
protozoan increasingly recognised as an important human pathogen. The ability to
detect Cryptosporidium in apple juice is required,
both in the context of investigations during an outbreak situation, and during
periods of surveillance or routine testing of apple juice for the presence of
pathogens. The objective of this study is to identify detection methods for
Cryptosporidium with the most promise for accuracy
and rapid results.
Project Identification: SA0202
CFIA0236
Establishment of accurate and rapid tests for food- and water-borne parasites:
Cyclospora, Giardia,
and Toxoplasma
Project leader: Alvin Gajadhar, Canadian Food Inspection Agency-Saskatoon,
agajadhar@inspection.gc.ca
Starting date: 2002/04
Description: Objectives of this study are to identify accurate and rapid
techniques for detection of the food- and water-borne parasites Cyclospora, Toxoplasma and
Giardia; establish standardized protocols for the
diagnostic tests identified for Cyclospora,
Toxoplasma and Giardia; and determine the detection limits and analytical
specificity for these diagnostic tests with purified parasites.
Project Identification: SA0203
CFIA0237
Standardization and validation of immunohistochemical and polymerase chain
reaction assays for Cysticercus bovis
Project leader: Brad Scandrett, Canadian Food Inspection Agency-Saskatoon,
bscandrett@inspection.gc.ca
Starting date: 2002/04
Description: Canadian Food Inspection Agency has recently developed an
immunohistochemical and a polymerase chain reaction assay for postmortem
diagnosis of Cysticercus bovis, the intermediate
stage of the human tapeworm Taenia saginata, in
cattle. Preliminary data suggest that these assays are more sensitive and
specific than histological evaluation. This study will compare the three assays
and provide evidence in support of, or against, adoption of the
immunohistochemical and PCR
assays for routine diagnostic use.
Project Identification: SA0205
CFIA0243
Development of analytical methodology for the determination of synthetic
pyrethroid residues in tissue of slaughtered animals and in milk and dairy
products
Project leader: Christine Akre, Canadian Food Inspection Agency-Saskatoon,
akrec@inspection.gc.ca
Starting date: 2000/04
Description: Synthetic pyrethroids are among the most widely used classes of
insecticides for plant protection. Persistent residues have been reported in
livestock, and routine monitoring programs in other countries have found
detectable residues of various pyrethroids in fat. This study will adapt,
validate and test a multi-residue method for pyrethroid residues in fat to
monitor for residues in domestic and imported meat. This project selected a
method that has been used by United States Department of Agriculture, Food
Safety and Inspection Service, for testing. An assessment of the method in its
original form is under way.
Project Identification: SF0001
CFIA0247
Improved test capability for banned substances in food of animal
origin
Project leader: JoeBoison, Canadian Food Inspection Agency-Saskatoon,
jboison@inspection.gc.ca
Starting date: 2000/04
Collaboration: Western College of Veterinary Medicine
Description: Continuing improvement of test capability for banned substances
in food of animal origin, this project will cover four distinct groups of drugs
that are either banned in Canada or by our trading partners, or have not been
approved for use in food animals by Health Canada. This study will investigate
Canadian Food Inspection Agency test capabilities equivalent to the European
Union, to detect and analyze banned substances in the residue control
program.
Project Identification: SF0103
CFIA0248
Development and implementation of analytical methods for the determination of
carbadox and five other feed additives (spriamycin, tylosin, virginiamycin,
bacitracin and olaquindox) banned by the European Union for use as antimicrobial
and growth promotants (AGPs) in food animal
production
Project leader: JoeBoison, Canadian Food Inspection Agency-Saskatoon,
jboison@inspection.gc.ca
Starting date: 2000/04
Collaboration: Western College of Veterinary Medicine
Description: This study will adapt published methods to determine the feed
additives virginiamycin and olaquindox, and develop a method to determine
bacitracin residues in animal tissues. The enhanced test capability will afford
an increased level of protection to Canadian consumers by contributing to
Canadian Food Inspection Agency's residue control program.
Project Identification: SF0104
CFIA0251
Development and implementation of multi-residue analytical methods for the
determination of Beta-lactams and phenicols in animal
derived foods
Project leader: Craig Salisbury, Canadian Food Inspection Agency-Saskatoon,
csalisbury@inspection.gc.ca
Starting date: 2002/04
Description: This project will focus on the development of methods to
determine residues of phenicols (thiamphenicol and the recently-registered
florfenicol) and Beta-lactams(amoxicillin, ampicillin, and
cloxacillin) in animal tissues, dairy products and egg products and on the
application of these methods to a pilot survey of field samples from the
antibiotic survey program.
Project Identification: SF0201
CFIA0252
Development/ implementation of analytical methods for macrolide and
aminoglycoside antibiotics in foods of animal origin
Project leader: Les Dickson, Canadian Food Inspection Agency-Saskatoon,
dicksonl@inspection.gc.ca
Starting date: 2002/04
Description: A series of multi-residue methods to determine selected
antibiotic compounds (macrolides and aminoglycosides) in animal tissues, milk
and dairy products, and eggs and egg products will be developed or adapted from
literature methods. The validated methods will be incorporated into diagnostic
programs to address the need for monitoring these residues in domestic and
imported meat, and products of animal origin.
Project Identification: SF0202
CFIA0253
Development/ implementation of analytical methods for the detection of
prednisone and other corticosteroids in meat and meat products
Project leader: Rick Fedeniuk, Canadian Food Inspection Agency-Saskatoon,
rfedeniuk@inspection.gc.ca
Starting date: 2002/04
Collaboration: Canadian Food Inspection Agency: Winnipeg Laboratory (Foreign
Animal Diseases)
Description: Objectives are to develop and validate an analytical method(s)
to determine prednisone (a synthetic glucocorticosteroid that has been used as a
growth-promoter in animal production and can mask signs of disease in animals
prior to slaughter) and its metabolites to 500 parts per trillion in meat and
meat products; develop liquid chromatography mass spectrometry/mass spectrometry
confirmatory ability for prednisone and relevant metabolites; and determine
current state of prednisone use in food markets.
Project Identification: SF0203
CFIA0257
Evaluation of high through-put, rapid tests for bovine spongiform
encephalopathy
Project leader: Stefanie Czub, Canadian Food Inspection Agency-Saskatoon,
Czubs@inspection.gc.ca
Starting date: 2002/04
Description: The purpose of the study is to prepare the implementation of a
high through-put bovine spongiform encephalopathy assay. The situation might
occur where rapid and high through-put tests are required to increase the level
of monitoring of Canadian cattle, especially should a positive animal be
detected in Canada or the United States by immunohistochemistry or
histopathology.
Project Identification: W0206
CFIA0258
Enhancement and implementation of the Canada Database of Animal
Parasites
Project leader: Lydden Polley, Canadian Food Inspection Agency Saskatoon,
lpolley@inspection.gc.ca
Starting date: 2002/04
Collaboration: Western College of Veterinary Medicine
Description: The Canada Database of Animal Parasites contains records of the
occurrence of twenty important helminth and protozoan parasites assembled from
the published literature as far back as possible, and from the veterinary
diagnostic laboratories. The focus this year will be on continuing to update the
database from both publications and diagnostic laboratories (including data
validation), developing the parasite profiles or providing links to pre-existing
sources of information and using available mechanisms to make CDAP more accessible
within the CFIA.
Project identification: SA0201
CFIA0301
Mass spectrometric verification of the endectocides doramectin, moxidectin,
abamectin, ivermectin, and eprinomectin in milk
Project leader: David A. Durden, Canadian Food Inspection Agency Calgary,
durdend@inspection.gc.ca
Starting date: 2003/01
Description: This project is aimed at developing an LC
MS/MS method for the confirmation of endectocide antiparasitic drugs
in milk. This method is intended to provide verification for milk samples which
prove positive for the above endectocides.
Project identification: CF0302
CFIA0302
Development/ implementation of a multi-residue confirmatory method for the
determination of Beta-lactams or penicillins in eggs and
milk using LC
MS/MS
Project leader: Jiang Wang, Canadian Food Inspection Agency Calgary,
wangj@inspection.gc.ca
Starting date: 2003/04
Collaboration: CFIA Saskatoon Laboratory -
Veterinary Drug Residues
Description: This study is designed to develop LC
MS/MS methodology to confirm the identity and concentration of
Beta-lactams in shell egg and raw milk samples.
Project identification: CF0303
CFIA0303
Development/ implementation of a multi-residue confirmatory method for the
determination of macrolides in eggs and milk using LC
MS/MS
Project leader: Jiang Wang, Canadian Food Inspection Agency Calgary,
wangj@inspection.gc.ca
Starting date: 2003/04
Collaboration: CFIA Saskatoon Laboratory
Veterinary Drug Residues
Description: Macrolides in eggs and milk are currently being screened using
the Charm II test in CFIA. Any positive samples
need to have the presence of macrolides confirmed. The present study is to
develop LC
MS/MS methodology to confirm the identity of macrolides in shell egg
and raw milk samples.
Project identification: CF0304
CFIA0304
Development/ implementation of analytical methods for the detection of specific
fungicides in fruit and vegetable commodities
Project leader: Donna Grant, CFIA Calgary,
grantd@inspection.gc.ca
Starting date: 2003/04
Description: Analytical methods for residues from specific fungicides in
fruit and vegetable commodities will be developed, validated and implemented
into ongoing monitoring and surveillance regulatory programs within the CFIA Calgary Laboratory.
Project identification: CF0305
CFIA0305
Development of HPLC/MS
methodology for the determination of azaspiracids in shellfish
Project leader: Garth Burns, Canadian Food Inspection Agency Dartmouth,
burnsbg@inspection.gc.ca
Starting date: 2003/04
Description: This project is designed to develop methodology to analyse for
toxins, hopefully within a multi-toxin analysis that will include DSP (diarrhetic shellfish
poisoning )and pectenotoxins. The method will allow for monitoring of the toxins
to provide early warning of contamination or to monitor outbreaks. The method
will be presented to the scientific community upon completion.
Project identification: D0301
CFIA0306
Development of HPLC/MS
methodology for the determination of nitrofuran metabolites in aquacultured
products
Project leader: Garth Burns, Canadian Food Inspection Agency Dartmouth,
burnsbg@inspection.gc.ca
Starting date: 2003/04
Description: Nitrofuran antibiotics have proven to be a problem because their
metabolites persist in host tissue, and as a result of their promotion of
mutagenicity, genotoxicity and chromosome damage. These antibiotics are used to
combat bacterial infections in aquacultured products in a number of countries,
and as a result their residues can be expected to be found in aquacultured
product being imported into Canada. It is proposed that a method in use by the
RIKILT Institute in the Netherlands be modified for use within this laboratory.
Once the method is validated it will be transferred to other CFIA laboratories and will be
available to industry, academia and the scientific community. It is expected
that the methodology will eventually be used in a monitoring program.
Project identification: D0302
CFIA0307
Carbon monoxide detection in CO treated fish flesh
Project leader: Dave McLachlan, Canadian Food Inspection Agency Dartmouth,
mclachlandg@inspection.gc.ca
Starting date: 2003/04
Description: In recent years carbon monoxide (CO) and "tasteless smoke"
(filtered smoke with a high percentage of CO present) have been administered by
fish processors in several off-shore countries to several fish species to
preserve and enhance flesh colour. At present, carbon monoxide treatment is an
unapproved process in Canada, therefore CO treated product is not permitted to
be imported into Canada. This study is to develop a quantitative assay which can
be conducted in any laboratory with the use of simple plastic/ glassware and a
relatively inexpensive ($500) digital carbon monoxide specific monitor
(sensitivity 0 1parts per million to 999parts per million). The analysis
would allow for the effective monitoring of suspect product entering the
country, with a minimum cost in materials and analysis time.
Project identification: D0303
CFIA0308
Functional foods containing fish oils: Correlating sensory analysis with
volatile odour compounds
Project leader: Dave McLachlan, Canadian Food Inspection Agency Dartmouth,
mclachlandg@inspection.gc.ca
Starting date: 2003/04
Collaboration: industry partner
Description: A primary objective in this proposal is to utilize the CFIA expertise in sensory
analysis to identify marker compounds which are characteristic odours caused by
oil rancidity or oxidation, as well as due to interactions with the food matrix.
Results will be used to establish sensory product standard guidelines for
regulatory use to ensure consumer safety when used in functional foods.
Project identification: D0304
CFIA0309
Development and optimization of a NASBA "real-time"
platform for the detection of hepatitis and Norwalk-like viruses (NLVs) in foods
Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency
St.Hyacinthe, trottieryl@inspection.gc.ca
Starting date: 2003/04
Collaboration: Agriculture and Agri-Food Canada: Food Research and
Development Centre, St.Hyacinthe; industry partner
Description: This project aims to increase the detection sensitivity of
food-borne viruses (hepatitisA and Norwalk-like viruses) and to develop a
quantitative assay for the detection of hepatitisA and Norwalk-like Viruses
(NLVs) in fruits and vegetables
(spiked blueberries and lettuce).
Project identification: H0302
CFIA0310
Development, optimization and validation of a new PCR and culture procedures for the
detection and isolation of Shigella species in
fresh produce
Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency
St.Hyacinthe, trottieryl@inspection.gc.ca
Starting date: 2003/04
Collaboration: Health Canada: Food Directorate, Ottawa
Description: Food-borne diseases associated with Shigella species are becoming an increasing risk to human
health in developed countries such as United States and Canada. Minimally
processed foods, such as fresh vegetables and salads, have been increasingly
implicated in outbreaks. This study will focus on the optimization and the
validation of the current PCR procedure for the detection of Shigella
in fresh produce, used by the United States Food and Drug Administration
Centre for Food Safety and Applied Nutrition. This methodology has proven to be
effective for the detection of all Shigella
species and Entero-Invasive Escherichia coli
(EIEC). This project
is being accomplished in collaboration with Health Canada scientists in order to
comply with the requirements of the Compendium of Analytical
Methods.
Project identification: H0303
CFIA0311
Development, optimization and validation of molecular methods for the detection
of enteric viruses in bottled water and berries
Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency
St.Hyacinthe, trottieryl@inspection.gc.ca
Starting date: 2003/04
Collaboration: Agriculture and Agri-Food Canada: Food Research and
Development Centre, St.Hyacinthe
Description: Recent food-borne outbreaks of acute viral gastroenteritis in
Canada and the United States have occurred through commodities such as water,
fresh fruits and vegetables (strawberries, raspberries, salads) and shellfish.
Norovirus (Norwalk-like viruses) and hepatitisA viruses have been responsible
for the majority of these outbreaks. Although several molecular detection
methods have been published, only a handful of methodologies are available to
extract the viruses from selected food matrices (such as berries) and bottled
water. The objective of this study is to provide an efficient extraction method
to remove the viral particles from bottled water and berries, removing
inhibitors and interfering substances, followed by a concentration step and
detection using optimized molecular detection methods.
Project identification: H0304
CFIA0312
Confirmatory tests to corroborate milk allergen detection in various food
matrix
Project leader: Philippe Raymond, Canadian Food Inspection Agency
St.Hyacinthe, raymondp@inspection.gc.ca
Starting date: 2003/04
Collaboration: Canadian Food Inspection Agency Longueuil; Health Canada:
Allergen Method Development Laboratory (Ottawa)
Description: Objectives are to develop and evaluate different confirmatory
methodologies for the detection of milk allergens adapted to the food matrix
composition. Cookies, chocolate bars, frozen yogurt are representative
"milk-free" food where hidden milk allergens were reported. Researchers will
further purify and characterize milk proteins and develop new milk protein
reagents and antibodies to improve our testing capacity. These approaches will
then be compared to the standard milk allergen ELISA for the different food
matrix types. Researchers expect to select the most appropriate confirmatory
test for hidden milk allergens and the information gathered from testing the
different matrix types would be highly valuable or directly transferable to
other allergens in similar matrices.
Project identification: H0305
CFIA0313
Detection of emerging zoonotic enteric viruses potentially transmitted through
livestock and food
Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency
St.Hyacinthe, trottieryl@inspection.gc.ca
Starting date: 2003/04
Collaboration: Agriculture and Agri-Food Canada: Food Research and
Development Centre, St.Hyacinthe
Description: The main objective of this project is to detect emerging
zoonotic enteric viruses in livestock, i.e. cattle, sheep and swine. Noroviruses
(Norwalk, Norwalk-like), the TT virus, the bovine Newbury Agent-2 and the
hepatitisE virus will be targeted. Molecular methods with primers specific to
the targeted viruses will be used to detect the presence of the viruses in the
feces of selected animals in abattoirs and on farms. The genomic portions
amplified using molecular methods will be sequenced and compared with existing
databases so that Canadian sequences can be compared with those from other
countries.
Project identification: H0306
CFIA0314
Evaluation of the suitability of a low density array method using a universal
sample preparation procedure for the detection of food-borne viruses using
DNA and RNA enteric viruses as models
Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency
St.Hyacinthe, trottieryl@inspection.gc.ca
Starting date: 2004/01
Collaboration: Agriculture and Agri-Food Canada: Food Research and
Development Centre, St.Hyacinthe; industry partner
Description: Food-borne viruses are an increasing concern in Canada and the
United States. Although viruses do not multiply in foods, they are, nonetheless,
resistant to environmental storage conditions and common practices for
controlling bacterial contamination. Since only a few viral particles can become
a risk to the consumer, one of the main challenges facing diagnostic
laboratories is to be able to concentrate these viral particles from the
suspected foods, in order to increase the probability of detection by molecular
methods and eliminate inhibitors that may interfere with the amplification
process. The objective of our project is to evaluate a "universal extraction/
concentration" procedure applicable to both viruses and bacteria from various
spiked food matrices (a "one step multi-pathogen" procedure). Rotavirus typeA
and Adenovirus40-41 have been selected as a model for RNA virus and DNA virus respectively.
Project identification: H0307
CFIA0315
Evaluation and validation of the NASBA amplification
approach for the detection of enteroviruses in food samples
Project leader: Carole Simard, Canadian Food Inspection Agency
St.Hyacinthe, simardc@inspection.gc.ca
Starting date: 2003/02
Collaboration: University of Montreal: Faculty of Veterinary Medicine;
Université Laval: Département des sciences des aliments et de nutrition;
Agriculture and Agri-Food Canada: Food Research and Development Centre
Description: The objective of this project is to compare various approaches
for the extraction and concentration of enteroviruses from various food samples,
as a preliminary step to detection by molecular methods. We will compare various
approaches for the recovery of hepatitisA virus and rotavirus from various food
samples, characterize the extraction-amplification method developed, and
validate the method by the analysis of samples of seafood, fruits and
vegetables.
Project identification: H0311
CFIA0316
Rapid detection and reduction of Salmonella
Typhimurium DT104 in poultry
using bacteriophage
Project leader: Jiewen Guan, Canadian Food Inspection Agency Ottawa
(Fallowfield), guanj@inspection.gc.ca
Starting date: 2003/04
Collaboration: Canadian Institute for Food Safety Research; Department of
Food Science, University of Guelph; industry
Description: In this study, we will investigate the development of a rapid
detection method for screening Salmonella
Typhimurium DT104. The method
is expected to be sensitive and specific, and will incorporate selective
enrichment and immunomagnetic separation. The entire detection procedure is
estimated to be completed within 36hours, and will provide a rapid alternative
to time consuming and labour intensive conventional culture methods. In
addition, we will also investigate the feasibility of using phage to reduce the
load of Salmonella Typhimurium DT104 in experimentally infected chickens.
The study will provide information on the potential use of phage as an
alternative to antibiotics for controlling multi-drug resistant pathogens in
agriculture.
Project identification: NAV0320
CFIA0317
Development of a novel, rapid and inexpensive method for epidemiological
analysis of Listeria monocytogenes and Escherichia coli strains isolated from clinical cases and
contaminated food during disease outbreaks
Project leader: Min Lin, Canadian Food Inspection Agency Ottawa
(Fallowfield), linm@inspection.gc.ca
Starting date: 2003/04
Collaboration: Queen University: Centre for Water and the Environment.
Description: This study is designed to develop a novel, rapid, simple and
inexpensive tool for epidemiological analysis of two important food-borne
pathogens Listeria monocytogenes and Escherichia coliO157:H7. The tool will be used by food
safety labs to rapidly trace back the sources responsible for outbreaks and
sporadic cases in epidemiological studies. It will also allow for the accurate
detection, identification and quantification of those Listeria
monocytogenes strains and Escherichia
coliO157:H7 in the food chains that pose the greatest risk to human
health.
Project identification: NBR0320
CFIA0318
Evaluation of postmortem diagnostic tests for confirmation of chronic wasting
disease in clinically affected and preclinical elk and deer
Project leader: Arumuga Balachandran, Canadian Food Inspection Agency
Ottawa (Fallowfield), balachandrana@inspection.gc.ca
Starting date: 2003/04
Collaboration: Animal Disease Research Unit (ADRU), United States Department
of Agriculture, Pullman, Washington; United States Department of Agriculture,
Ames, Iowa; Colorado State University
Description: Any eradication program for disease in livestock is dependant on
the validity of the diagnostic criteria. The critical issue in relation to
chronic wasting disease is the deficit of information on possible correlations
between various diagnostic tests available. Commercial firms in Europe are
developing based tests for detection of the scrapie prion protein (PrPSc) in brain tissue of
TSE suspected
animals. The United States Department of Agriculture and CFIA are collaborating with
these groups to evaluate the tests as large-scale screening and surveillance
tools.
Project identification: NTSE0101
CFIA0319
Development of a bioassay to detect infectivity in tissues and body fluids of
elk and deer naturally infected with chronic wasting disease
Project leader: James Algire, Canadian Food Inspection Agency Ottawa
(Fallowfield), jalgire@inspection.gc.ca
Starting date: 2003/04
Description: The main aim of this project is to establish a bioassay system
to measure the infectivity in the various tissues and products of chronic
wasting disease infected animals, and to aid in the diagnosis of chronic wasting
disease in subclinically infected (carrier animals) not showing signs of
disease.
Project identification: NTSE0204
CFIA0320
Preliminary investigation of host response patterns in chronic wasting disease
and scrapie as disease markers for preclinical diagnostic tests
Project leader: Hongsheng Huang, Canadian Food Inspection Agency Ottawa
(Fallowfield), huangho@inspection.gc.ca
Starting date: 2003/04
Description: While the methods to diagnose transmissible spongiform
encephalopathies (TSEs) including
chronic wasting disease and scrapie have improved tremendously over the past
decade, early detection in living animals remains problematic. The objective of
this study is to catalogue a series of blood-borne markers associated with
TSEs with a
view to recognize a specific response pattern to discriminate diseased animals
from healthy animals. Presence of a specific response pattern (or lack of it) at
specific time points during incubation and clinical disease may assist in
recognizing carrier animals.
Project identification: NTSE0304
CFIA0321
Development of ultrasensitive immuno-PCR and enzyme immuno-fluorescence
techniques for food testing: detection of bacterial toxins and quantitation of
peanut allergen in foods
Project leader: Burton W. Blais, Canadian Food Inspection Agency Ottawa
(Carling), bblais@inspection.gc.ca
Starting date: 2003/04
Collaboration: Health Canada: Bureau of Microbial Hazards; Health Canada:
Guelph, Ontario; Canadian Food Inspection Agency-Ottawa (Fallowfield);
industry
Description: This project addresses the development and evaluation of
ultrasensitive fluorescence-based assay systems as novel technology platforms
for the detection of priority analytes in foods. To demonstrate the broad
applicability of these technologies this study will focus on the development of
applications targeting analytes for the detection of bacterial toxins and food
allergens. Test systems will be developed and evaluated for the detection of the
priority toxins botulinum neurotoxin (BoNT, typeE) and verotoxin (VT2), and for the detection and quantification of
peanut protein in foods. The performance characteristics of the assay system
showing the greatest promise will be further evaluated to assess its
suitability. This assay format will also be adapted to the detection of VT2.
Project Identification: OLC0301
CFIA0322
Validation of ELISA
for antemortem diagnosis of Cysticercus
bovis-infected cattle
Project leader: Dele Ogunremi, Canadian Food Inspection Agency Saskatoon,
dogunremi@inspection.gc.ca
Starting date: 2003/04
Description: Cattle infected with the tapeworm cyst Cysticercus bovis are difficult to detect at meat inspection
because most infected animals harbour only a few cysts. Sensitive antemortem
detection based on the detection of antibodies in bovine serum should help in
minimizing the costs of managing infected herds by providing a tool that will
enable the focussing of intervention strategies on infected cattle only. The aim
of this project is to standardize and validate an antibody detection ELISA for the diagnosis of
Cysticercus bovis. Banked serum samples obtained
from past investigations of both experimental and natural infected cattle will
be used for analysis, as will negative bovine serum samples. Cattle infected
with other helminths will be included as controls. It is expected that at the
end of the study, test parameters will be available to support the use of the
test for antemortem detection of Cysticercus
bovis-infected cattle.
Project identification: SA0304
CFIA0323
Improved test capability for hormones and other growth promoting substances in
urine and tissues
Project leader: Jim MacNeil, Canadian Food Inspection Agency Saskatoon,
jmacneil@inspection.gc.ca
Starting date: 2001/04
Description: This project was established to develop and implement tests to
expand the scope of testing for growth promoting substances. The tests will meet
method validation requirements of revised European Union directives. Methods
under investigation include liquid chromatography, gas-chromatography (GC/MS) and LC
MS/MS.
Project Identification: SF0101
CFIA0324
Development/ implementation of a multi-residue analytical method to determine
beta-lactams in honey using liquid chromatography/ electrospray ionization
tandem mass spectrometry
Project leader:Jiang Wang, Canadian Food Inspection Agency Calgary,
wangj@inspection.gc.ca
Starting date: 2002/04
Description: The beta-lactam antibiotics are used to treat infectious human
and animal diseases and to enhance growth and yield in agriculture. Maximum
residue limits for beta-lactam antibiotics drug residues in some food
commodities, such as milk and eggs, have been established by Health Canada, the
European Union and the United States Food and Drug Administration Center for
Veterinary Medicine. This project will adapt and validate a multi-residue
analytical method for beta-lactams in honey and transfer the method to the
analytical testing staff at the Calgary Laboratory.
Project Identification: CF0201
CFIA0325
Development/ implementation of analytical method to determine aminoglycosides in
honey
Project leader: Dugane Quon: Canadian Food Inspection Agency Calgary, quond@inspection.gc.ca
Starting date: 2002/04
Description: CFIA
has the capability to screen for aminoglycosides (streptomycin residues) in eggs
and milk. There is a concern that streptomycin residues may be present in honey,
particularly in imported honey. This project will allow the CFIA to expand its capability
to analyze and confirm the presence of streptomycin residues in honey.
Project Identification: CF0203
CFIA0326
Development/ implementation of analytical method for the determination of
tetracyclines in egg and egg products
Project leader: David A. Durden, Canadian Food Inspection Agency Calgary, durdend@inspection.gc.ca
Starting date: 2002/04
Description: One of the major groups of antibiotics is that of tetracyclines.
The CFIA has
quantified tetracyclines in a variety of food stuffs, and amethod has been
developed in Calgary for tetracycline residues (oxytetracycline, tetracycline,
chlortetracycline) in honey. Objectives are to adapt and validate a residue
method for tetracyclines in eggs and egg products by LC/MS, to add marker
compounds to the LC/MS residue method
and to transfer the methods to Calgary analytical testing staff.
Project Identification: CF0209
